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Bioanalyzer Assays

The RNA 6000 Nano LabChip and the DNA 1000 LabChip are commonly used by the Core. Information for these LabChips is listed here. Information regarding additional LabChips with different sizing and sensitivities are also available directly from Agilent. The Core will train investigators to run the Bioanalyzer themselves.


RNA 6000 Nano

    12 samples per chip
    Sample volume: 1 ul
    Analysis run time: 30 minutes
Specifications
  Total RNA Assay mRNA Assay
Quantitative range 25 - 500 ng/ul 25 - 500 ng/ul
Qualitative range 5 - 500 ng/ul 25 - 250 ng/ul
Maximum sample buffer strength 100 mM Tris or
125 mM NaCl or
15 mM MgCl2
100 mM Tris or
125 mM NaCl or
15 mM MgCl2



DNA 1000

    12 samples per chip
    Sample volume: 1 ul
    Analysis run time: 35 minutes
Specifications
  DNA 1000 Assay
Sizing range 25 - 1000 bp
Sizing resolution ±5 bp for 25-100 bp
±5% for 100-1500 bp
±10% for 1500-7500 bp
Sizing accuracy ±10%
Quantitation accuracy ±20%
Concentration range 0.1 - 50 ng/ul
Maximum salt concentration per sample 125 mM for KCl or NaCl
15 nM for MgCl2

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